Methods in Enzymology. Volume 207

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This book describes modern electrophysiological techniques for recording and analysis of ion channels in various systems including cells in culture, Xenopus oocytes, brain slices, cellular organelles, and microorganisms. Many chapters deal with the prevention and correction of electrophysiological artifacts. Also included are methods for the purification and expression of ion channel genes and proteins.Key Features* Applications of electrophysiological techniques* Expression in heterologous systems* Reconstitution of lipid bilayers* Purification and characterization of ion channel proteins and genes* Recording of ion channels of intracellular membranes and microorganisms* Data storage and analysis