Shan S. Wong Livres

Focusing on the chemical principles of cross-linking and conjugation, this book provides an extensive compilation of cross-linking reagents documented since the first edition. It categorizes these reagents into monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers. Additionally, it reviews their applications, making it a valuable resource for researchers in the field.

Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, Chemistry of Protein Conjugation and Cross-Linking discusses the mechanism of reaction and allows you to put the theory into practice. The book offers an explanation of the underlying mechanism of chemical modification, surveys all the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents in various areas of biochemistry, molecular biology, biotechnology, nucleic acid chemistry, immunochemistry, and diagnostic and biomedical disciplines. It contains numerous examples and illustrations, plus step-by-step explanations to reaction procedures. It is an excellent introduction and a comprehensive reference about chemical modification.