Insights into protein dynamics by time-resolved x-ray scattering

Rohit Jain, born 1983 in Delhi, India, performed his PhD thesis at Max Planck Institute for Biophysical Chemistry, Goettingen from 2011 until 2015. The goal was to reach a better understanding of protein dynamics with time-resolved X-ray scattering technique. It was achieved by working on three complementary themes. They are viz., (a) to develop a new real-time 20-microchannelrapid-mixing time-resolved small-angle X-ray scattering (TR-SAXS) method; to investigate the early millisecond steps of ubiquitin unfolding and to quantitatively describe the transient ubiquitin unfolding ensembles by using this novel methodology; (b) to obtain insights into the conformational structural dynamics of the enzymatic cycle of human guanylate kinase (hGMPK) with X-ray scattering and (c) laser pump-X-ray scattering probe experiments to probe ubiquitin dynamics on picosecond to millisecond timescale.